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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is observed in lots of tumors, which ends up in chromosome instability, and thus may well lead to tumorigenesis. This protein was located to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a critical role in promoting mobile-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been explained. Two more splice variants have been noted but in depth nucleotide sequence info is not really still obtainable.
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Blocking peptides are peptides that bind precisely for the focus on antibody and block antibody binding. These peptide ordinarily consists of the epitope identified via the antibody.
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This guideline outlines additional feasible optimization of cellular and immunoassay parameters to make certain the absolute best benefits are...
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This manual outlines even further achievable optimization of mobile and immunoassay parameters to guarantee the absolute best benefits are...
Antibodies bound to the blocking peptide no more bind towards the epitope over the goal protein. By click here evaluating the staining in the blocked antibody as opposed to the antibody alone, one can see which staining is unique.
Goal Information and facts Cyclin E belongs for the remarkably conserved cyclin family members, whose customers are characterised by a extraordinary periodicity in protein abundance with the cell cycle. Cyclins purpose as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute for the temporal coordination of each mitotic function. Cyclin E kinds a fancy with and functions like a regulatory subunit of CDK2, whose action is required for cell cycle G1/S changeover. This protein accumulates at the G1-S section boundary and is particularly degraded as cells development by S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by equally antibodies...
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